Bio-Rad Affi-Gel Blue Gel - Manual (Page 7)

is so strong that a high concentration of salt or chaotropic
reagent is required to desorb the albumin. Other serum
proteins either do not bind to Affi-Gel blue gel or can be
eluted with relatively low concentrations of salt.
Table 1. Buffer Formulations For Albumin
Removal Procedure
A Buffer 20 mM phosphate buffer, pH 7.1
B Buffer 1.4 M NaCl, in 20 mM phosphate buffer,
pH 7.1
C Buffer 2 M guanidine HCl in 20 mM phosphate
buffer, pH 7.1
or 1.5 M NaSCN in 20 mM phosphate buffer,
pH 7.1
Procedure
1. Prepare a column of Affi-Gel blue gel, 50-100 mesh,
with a total bed volume of 5 ml of gel per milliliter
of serum to be processed.
2. Prewash the column with 2 bed volumes of buffer A.
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