Bio-Rad Affi-Gel Blue Gel - Manual (Page 8)

3. Equilibrate the serum sample in buffer A by
dialyzing overnight, or by rapid column desalting in
a column of Bio-Gel P-6 DG gel, on an Econo-Pac
10DG column, or on an Econo-Pac P6 cartridge.
4. Apply the equilibrated serum sample to the column.
5. Wash the column with 2 bed volumes of buffer A.
The effluent from this step contains the serum
proteins minus most of the albumin.
6. Optional step: elute the albumin with buffer B.
7. Whether or not the albumin was eluted, regenerate
the column with 2 bed volumes of buffer C.
3.3 Enzyme Purification
Affi-Gel blue gel has been used to purify a number
of enzymes. It has been particularly useful in the
purification of kinases, dehydrogenases, and other
nucleotide-dependent enzymes. The degree of
purification obtained with Affi-Gel blue gel is typically
much greater than that obtained using biospecific
affinity chromatography. It has been suggested that
enzymes containing a “dinucleotide fold” bind
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