Bio-Rad Affi-Gel Blue Gel User Manual
Page 12
Table 2. Affi-Gel Blue Gel Enzyme Applications
(continued)
Enzyme
Source
Eluant
Reference
Glutamine synthetase
Salmonella typhimurium
5 mM ATP
8
Glyoxalase II
Rat erythrocytes
0-0.2 M KCl
19
GMP reductase
Human erythrocytes
1 mM NADPH, 2 mM GMP
21
GTP:RNA guanylyltransferase
Wheat germ
0.05-0.75 M NaCl
5
Isocitrate dehydrogenase
E. coli
2 mM NADP
16
Isocitrate dehydrogenase
E. coli
2 mM NADP+
13
MB creatine kinase
Human heart
0.25 M NaCl
7
Membrane-bound
Rat brain
Triton X-100, NaCl, glycerol
38
phosphatidylinositol kinase
Methylenetetrahydrofolate
Porcine liver
0-10 mM NADPH
17
reductase
Metmyoglobin reductase
Bovine heart
1.0 M NaCl, 1 mM NADP+
11
Phosphodiesterase
Bovine brain
0.2 M NaCl
25
Replication protein A (RP-A)
Animal cells
-
40
RNA ligase
E. coli
0.2 M NaCl, 2 mM ATP
20
Serine transhydroxymethylase
Porcine liver
0.5 M KCl
18
Thymidylate
Saccharomyces cervisiae
0.25-1 M KCl
22
Tyrosine phenollyase
Erwinia herbicola
1.5 M NaCl, 0.5 mM mercaptoethanol
9
10
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