Bio-Rad Affi-Gel Blue Gel - Manual (Page 15)

7. Regenerate the column by washing it with 2 bed
volumes of 2 M guanidine HCl or 1.5 M NaSCN,
followed by 2 bed volumes of the starting buffer.
3.4 Purification of Blood Proteins
Affi-Gel blue gel has been used to separate and purify
a number of different serum and plasma proteins. Table 3
lists several examples. Gianazza and Arnaud
29,30 have
developed a single step method for general fractionation
of plasma proteins. By altering pH and ionic strength,
twenty-seven plasma proteins were separated, providing
better initial purification of individual proteins than many
of the two and three step methods commonly used.
Table 3. Purification of Blood Proteins on
Affi-Gel Blue Gel
Application Reference
Purification of human serum complement 26
Purification of rat alpha fetoprotein 27
Purification of rat serum albumin 28
Separation and purification of plasma proteins 29,30
Purification of alpha-2-macroglobulin 31
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