Bio-Rad Bio-Gel® HTP Hydroxyapatite - Manual (Page 10)

2. Swirl the buffer in the bottle gently until the gel is in
suspension. Do not use stirring rods or magnetic stir
bars.
3. After the gel is suspended, pour it into a beaker and
allow the gel to settle for at least 30 minutes. Decant
the fines which are in the cloudy upper level and at
the top of the settled bed. Decant to the settled bed.
Section 4
Pouring the Column
Many types of columns and bed supports are avail-
able. Glass barrel Econo-Column ® chromatography
columns* are useful for hydroxyapatite chromatography.
To pack the column, attach a wide mouth funnel to
the top of the column and add the starting buffer to it.
Then pour the suspended hydroxyapatite into the funnel
and allow 2-3 cm to settle under gravity. Then open the
column outlet, and allow the gel to pack under flow.
8
* For information on Econo-Column low pressure chromatogra-
phy columns, see Bio-Rad’s current catalog.
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