Bio-Rad Bio-Gel® HTP Hydroxyapatite - Manual (Page 4)

High capacity - Hydroxyapatite has a high capacity for
nucleic acids and proteins. Its surface area is about 50 m
2
per gram.
Low non-specific adsorption - Non-specific adsorption
of hydrophobic substances is minimized by the inorganic
crystalline matrix of hydroxyapatite [Ca
5
(PO
4
)3OH]
2
.
Hydroxyapatite displays negligible adsorptive capacity
for low molecular weight substances such as mononu-
cleotides, salts, and amino acids.
Chemical and thermal stability - The wide range of
chemical compatibilities (aqueous and inorganic sol-
vents), the thermal stability (autoclavable), and the pH
tolerance (pH >5.5) permit the use of hydroxyapatite
under conditions that optimize the binding of nucleic
acids and proteins.
Economy - The initial cost of the material is low, and can
be used several times.
Commercial hydroxyapatite preparations may vary
considerably in their ability to achieve the desired chro-
matographic resolution. All Bio-Rad hydroxyapatite is
tested for separation of double-stranded DNA from
2
LIT217C 6/17/98 1:52 PM Page 2