Bio-Rad Bio-Gel® HTP Hydroxyapatite - Manual (Page 7)

Table 1. Hydroxyapatite Product Performance
DNA Grade
Bio-Gel HT Bio-Gel HTP Bio-Gel HTP
Hydroxyapatite Hydroxyapatite Hydroxyapatite
Flow rate cm/h 25-100 35-100 >5
column cross section a
Mg BSA sorbed 10 10 10
per dry gram b
µg calf thymus DNA 500 500 800
sorbed per dry gram
Storage 4 °C dry form @ dry form @
room temp. room temp.
hydrated @ 4 °C hydrated @ 4 °C
a. Flow rate determined in a 1.5 x 10 cm column with 40 cm H20
hydrostatic pressure.
b. Batchwise uptake.
c. The DNA capacities listed are lower than previously reported
due to a change in testing methodology. This reporting change
does not represent diminished Hydroxyapatite product prefor-
mance or changes to the manufacturing process. The new testing
methodology results in a more accurate determination of DNA
capacity.
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