Bio-Rad Bio-Gel® HTP Hydroxyapatite User Manual
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single-stranded DNA, albumin binding capacity, DNA
binding capacity, and flow capacity. The test results for
ds DNA from ss DNA for each batch are printed on the
package label.
1.1 Bio-Gel HT Fully Hydrated
Hydroxyapatite
Bio-Gel HT hydroxyapatite, prepared by the method
of Tiselius,
et al.,
22
is shipped suspended in 10 mM sodi-
um phosphate buffer containing 0.02% NaN
3
. This mate-
rial gives excellent resolution at a high flow rate (see
Table 1) because of its large particle size. Bio-Gel HT
hydroxyapatite has a shelf life of at least 1 year when
stored at 4 °C in the shipping buffer.
1.2 Bio-Gel HTP Powder
Bio-Gel HTP hydroxyapatite is the Tiselius material
which has been dried by a unique process developed at
Bio-Rad. It may be stored without refrigeration, and,
when resuspended in buffer, it has the same properties as
Bio-Gel HT hydroxyapatite.
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