Bio-Rad Bio-Gel® HTP Hydroxyapatite - Manual (Page 12)

Section 6
Regenerating the Column
A 0.4 M phosphate buffer is usually sufficient to
remove adsorbed materials. If a contaminated sample has
been run, it is advisable to remove the top layer of the
hydroxyapatite bed and wash the remainder of the bed
with one bed volume of 1 M sodium chloride followed
by four column volumes of starting buffer.
For information on Bio-Rad's high performance
hydroxyapatite (Bio-Gel HPHT) columns, request bul-
letin 1115, or contact your local Bio-Rad representative.
In the U. S., call technical service at 1-800-4BIORAD.
Section 7
References
2 1. Stanker, L. D., Vanderlaan, M. and Juarez-Salinas, H., J.
Immunol. Methods, 76, 157 (1985).
2 2. Brooks, T. and Stevens, A., American Laboratory, 17, 54
(1985).
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