Bio-Rad Bio-Gel® HTP Hydroxyapatite - Manual (Page 8)

Section 2
Rehydrating Bio-Gel HTP and
DNA Grade Bio-Gel HTP
Hydroxyapatite
1. Determine the amount of dry powder needed to fill
the column. When hydrated, Bio-Gel HTP hydroxya-
patite occupies approximately 2-3 ml per dry gram.
2. Add one part Bio-Gel HTP hydroxyapatite or DNA
Grade HTP hydroxyapatite to six parts of starting
buffer with gentle swirling. Do not use magnetic stir
bars or stirring rods, as these will damage the hydrox-
yapatite crystals.
Note: All buffer should be degassed prior to the addition
of the dry hydroxyapatite.
3. Allow the slurry to settle for at least 10 minutes.
Then decant the fines which are in the cloudy upper
level and at the top of the settled bed. Decant to the
settled bed.
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