Bio-Rad Bio-Gel® HTP Hydroxyapatite - Manual (Page 9)

4. A second decantation is usually not necessary. If it is
desired, add an equal volume of starting buffer to the
bed and mix by swirling gently.
5. Repeat step 3 and resuspend a final time for column
pouring.
Section 3
Resuspending Bio-Gel HT
Hydroxyapatite
1. Bio-Gel HT hydroxyapatite is shipped in 10 mM
sodium phosphate buffer, pH 6.8, containing 0.02%
NaN
3
. It tends to pack in the bottle during shipping,
and requires resuspension for column pouring. If a
buffer other than phosphate is to be used in the actual
elution, it should also be used in the decanting steps.
Pour off the phosphate buffer in the bottle and add
the new buffer solution before suspending the gel.
Buffer pH should remain greater than 6.0.
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