Bio-Rad Ligation and Transformation Module - Manual (Page 3)

Table of Contents
Introduction ..................................................................................1
Kit Inventory Checklist ................................................................6
Safety Issues ................................................................................9
Background ................................................................................10
Quick Guide ................................................................................26
Instructor’s Advance Preparation ............................................32
Student Ligation Protocol..........................................................36
Student Transformation Protocol ..............................................40
Appendix A Inoculating a Bacterial Colony for
Plasmid Miniprep ................................................46
Appendix B Restriction Digestion of Plasmid DNA
with Bgl II Enzyme ..............................................48