Bio-Rad Ligation and Transformation Module - Manual (Page 55)

Restriction enzyme digestion analysis of plasmid DNA. Circular plasmid DNA purified from
bacterial minipreps is isolated and digested with Bgl II, a restriction enzyme producing at least
two linearlized fragments — vector DNA and PCR fragment (lane 2). These fragments can be
visualized using agarose gel electrophoresis. If the PCR fragment contains a Bgl II restriction
site, three DNA bands may be observed (lane 3). A 500 bp molecular weight ruler is shown in
lane 1.
BgI II
PCR
Fragment
PCR Fragment
BgI II
Bgl II
Digest
1 2 3
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