Bio-Rad Ligation and Transformation Module - Manual (Page 9)

When Activity to Complete Duration
Immediately following Transform E. coli with ligation 1 h
ligation or during the mixture and plate bacteria on
next laboratory activity LB Amp IPTG agar plates
Incubate transformed bacteria 16+ h
at 37°C
Next day after the Analyze results 0.5 h
transformation step Grow bacterial colony in LB Amp 16+ h
broth for miniprep
Day after growing Perform miniprep plasmid 1 h
bacterial culture for purification to isolate
miniprep plasmid carrying insert
Next laboratory activity Digest plasmid DNA with 1 h
Bgl II restriction enzyme
Analyze digest by agarose gel 1 h
electrophoresis
Prepare the DNA insert for 0.5 h
sequencing
Note: Bolded steps use reagents from the Ligation and Transformation
module.
5