Bio-Rad Ligation and Transformation Module - Manual (Page 35)

19. Retrieve the warm LP Amp IPTG
agar plate from the 37°C
incubator and pipet the entire
volume of the transformation onto
the labeled agar plate. Use an
inoculation loop to very gently
spread the bacteria around the
plate — do not spread for more
than 10 seconds.
20. Immediately place agar plate,
upside down at 37°C and
incubate overnight.
21. The next day, analyze results or
wrap plate in Parafilm and store
at 4°C until required.
31
37°C incubator