Bio-Rad Ligation and Transformation Module - Manual (Page 49)

23. Immediately place LB Amp IPTG agar plates upside down in the 37°C
incubator and incubate them overnight.
24. The next day, analyze the results, or wrap the plates in Parafilm and
place them at 4°C until required for inoculation of miniprep cultures
(see Appendix A).
Analysis of Results of Ligation and Transformation
Count the number of bacterial colonies that grew on the LB Amp IPTG
agar plates.
Note: Occasionally, satellite colonies may grow using this ligation method.
Count the large individual colonies, not the tiny colonies surrounding larger
colonies.
Transformation Number of Colonies
Ligation of gene of interest
Control plasmid
Note: If the number of colonies is very high and uncountable, enter "TNC"
for "too numerous" to count in the results table.
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