Hudson Robotics SOLO - Manual (Page 30)

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Increment Order – (applies only to aspirating from a plate position for
SOLO equipped with single tip pipettor head or multi-tip pipettor
head used in ‘Single Tip Mode’)
Row Order (disabled for multi-tip option of ‘P8’type syringe) : If a
multiple number of wells per execution is entered, or
if this step is within a Loop, each aspiration will increment in the
order “A1, A2, …. , A12, B1,…, B12, etc.”.
Column Order(disabled for multi-tip option of ‘P12’type syringe):
“A1, B1, … , H1, A2, …, H2, etc.”.
Reverse Order: wells with non-zero volumes are selected beginning at
H12 (for a 96-well plate) instead of A1. Row or Column order rules
apply, but in reverse.
Note: For multi-tip ‘P8’ syringe all non-zero volume wells in each
row will be addressed before moving to the next row.
Note: For multi-tip ‘P12’ syringe all non-zero volume wells in each
column will be addressed before moving to the next column.
Any wells with a zero volume will be skipped by single-tip units and
not included in the increment count.
Syringe (uL) –
This is read from the SOLO’s firmware and is selected from a
dropdown list of the syringes actually on this unit.
Start by Emptying Syringe (‘Pn’ syringe type only, where n is 0,1,8 or 12)
Checking this box will cause the needle or tip to move over the
intended aspirate position, then the syringe piston will move to its
bottom (empty) position at the start of this step. This is useful to
ensure that the full capacity of the syringe is available for this
aspiration. Otherwise the aspiration will start at the last position of the
syringe piston.
Find Bottom of Vessel or Well –
If this box is checked, the tip will travel down under low power until it
stalls on the bottom of the plate well or tube. Then, it will rise by the
distance in the Z Shift box under the ‘Aspirate Shift’ parameters.
Mixing –
If the box is checked, immediately after either dispence or aspiration,
the SOLO will dispense then aspirate the amount in ‘Mix Volume’, for
the number of times given by the ‘Mix Cycles’ value, ending with a
final dispense to leave the mixed sample in the well or vessel.
The aspirating action of the mixing will take place at the height
indicated by the ‘Z Shift’ entry, while the dispense action will occur at
the ‘Dispense Height’ distance from the well bottom (for plates)
or from the taught point.
Valve Port (‘D’ syringe type only, not shown) –
The user must select the appropriate fluid reservoir by choosing the
correct valve from a drop down list for the selected syringe pump.