Hudson Robotics SOLO - Manual (Page 44)

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target wells and dispense equal amounts in each until they receive the full amount
required. In order to reduce any effect from pipetting residual volumes at the very
lowest end of the pipette tip’s range, the next-to-last aspiration step will be limited
to 50% of the total remaining aspiration volume so that the last aspiration is also
at least 50% of the Max. Volume setting.
The user can browse the computer for the appropriate folder where Source plate
files will be found, and can browse or simply enter the full path and name of the
output file. Then, before saving the method, can select those Target plate rows,
columns or individual wells which will be blocked from receiving transfers from
the Source plate. These will be colored black as in the above graphic. The user
can also select whether to fill the Target plate in “Row” (across the top, then
down) or “Column” order. Pending but un-aspirated “hit” wells on the Source
plate are displayed in yellow, while completed transfers are shown in green on
both plates.
If the Source Plate’s “hits” have all been transferred, or if the Target Plate is full,
the HitPick Step will end, and, if this step is imbedded inside a Loop, that Loop
will end and the Method will proceed with the next step beyond that Loop’s
End Loop step. Usually, the Method will have been designed to end here to
enable replacing either of the finished plates.
Not shown in the graphic above are the aspiration and dispensing settings that the
HitPick Step has in common with the Aspirate and Dispense Steps, which are in
the lower portion of the HitPick Step’s graphic. Also, a ‘Mix’ option is provided
for both the Source and Target plate wells which functions identically to those in
the Aspirate and Dispense Steps, described in those sections.