Hudson Robotics SOLO User Manual
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The file for a 384-well plate would have 16 rows of 24 values each.
For multi-tip operation only values for wells addressed by first tip of
multi-tip head are taking in consideration. Tips on the head are
counted from left to right. See bellow file list for aspirating with 12
tip head (‘P12’) from 96-well plate:
50, 50, 50, 50, 50, 50, 50, 50, 50, 50, 50, 50
42, 42, 42, 42, 42, 42, 42, 42, 42, 42, 42, 42
0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0
0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0
0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0
0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0
20, 20, 20, 20, 20, 20, 20, 20, 20, 20, 20, 20
10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10
Well positions that have zero (or negative) volume values will simply
be skipped. The SOLO will aspirate from the number of non-zero
wells determined by the “Multiple Wells” setting, then, if inside a
“Loop” , will start at the well following the last one aspirated during
the previous execution of this step. The volumes to be aspirated will
appear in their respective well locations in the plate image across the
bottom of the display.
Aspirate from ‘Named’ Point:
The volume to be aspirated from a point, such as a vial location, can
also be imported from a comma-separated text file, but for this type of
position, the values can be in any number of rows/columns, and are
read in left-to-right, top-to-bottom order. As in the case of
aspirating from a plate position above, any zero (or negative) volume
values will simply be skipped. The SOLO will aspirate the next non-
zero volume in the file. If inside a “Loop”, it will use the value
following the last one aspirated during the previous execution of this
step. A complete list of the values to be aspirated can be viewed in the
dropdown’ list inside the “Aspirate Volume(uL)” box.