Hudson Robotics SOLO - Manual (Page 31)

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Syringe Speed –
Scroll up or down to accelerate or slow the syringe piston’s movement.
Pre-Aspirate –
Enter this volume to aspirate air before each aspiration event in this
step. This allows air pockets to separate each individual aspirated
sample, and also allows the dispense volume to “blowoff” with
an air pocket to forcibly expel tip droplets at the end.
Backlash –
Enter the number (the default is 0) of uL that you wish to have the
syringe piston move in reverse at the end of its stroke. This is used to
control droplets or end-of-tip air gaps (also used in a Dispense step) for
a given commanded volume.
Multiple Wells –
The number of aspirations done in each execution of this step, in the
selected row or column order. Any wells with a zero volume will be
skipped and not counted in the number selected.
Note: For multi-tip ‘P8’ only column order is available and for ‘P12’
syringe only row order is available .
Switch to File Data –
If the ‘Switch to File Data’ option is selected, the user will be
shown a browse screen to browse for the file or to just enter its name.
The selected file and its path will be shown just below the button’s
location. The text on the button will change to “Switch to Entered
Data” to enable the user to go back to using the plate image table
across the bottom of the display, and manually enter volume values for
each well or the named point. The file structure will be determined by
which type of position is to be aspirated from:
Aspirate from Plate Position:
The volume to be aspirated from each well can be imported from a
comma-separated text file with a row/column format that matches the
plate type selected for this position (e.g., 8 rows of 12 values each for a
96-well plate). This file lists each row on a separated line, with each
column’s value separated by a comma, resulting in a file similar to that
shown below for a 96-well plate:
50,58,66,74,82,90,98,106,114,122,130,138
51,59,67,75,83,91,99,107,115,123,131,139
52,60,68,76,84,92,100,108,116,124,132,140
53,61,69,77,85,93,101,109,117,125,133,141
54,62,70,78,86,94,102,110,118,126,134,142
55,63,71,79,87,95,103,111,119,127,135,143
56,64,72,80,88,96,104,112,120,128,136,144
57,65,73,81,89,97,105,113,121,129,137,145