ANKOM TDF Dietary - Manual (Page 43)

Operator’s Manual
Rev E 12/2/14
pg. 43
23.6 With your Heat Sealer set between 3 and 4 (settings may vary depending on the
heat sealer and the power source), seal each bag just above the filter to keep all
residue contained to the filter area while handling the bags.
23.7 Place each bag in the Drying Rack.
24. Dry the SDF filter bags.
24.1 Make sure your oven is 105°C at the location where the bags will be placed.
24.2 Place the Drying Rack with the filter bags in the oven and dry to constant weight (about 90 minutes).
24.3 When dry, remove all SDF filter bags from the oven and place them in a desiccant pouch to cool.
25. Weigh the TDF residue.
25.1 Remove one SDF filter bag from the desiccant pouch.
25.2 Roll or fold the bag, place it in a tared Bag Weigh Holder (BWH), and place the BWH in the center of a balance.
25.3 Record the weight of the filter bag.
25.4 Repeat steps 25.1 – 25.3 for each SDF filter bag used in the fiber analysis.
26. Determine the Protein content within the TDF residue.
See the “Protein Determination” section of this manual for recommended procedures.
27. Determine the Ash content within the TDF residue.
See the “Ash Determination” section of this manual for recommended procedures.
28. Calculate the % TDF value.
% TDF = [(R
1
+ R
2
)/2] - P - A - B
(M
1
+ M
2
)/2
= [(( f
F1
- f
S1
- D
1
) + ( f
F2
- f
S2
- D
2
))/2] - P
1
- (A
2
- D
2
) - B
(M
1
+ M
2
)/2
Where: M
1
, M
2
= Original wt of duplicate samples adjusted for pre-treatment fat and sugar losses (g)
R
1
, R
2
= Residue for duplicate samples (g)
f
F
= Final Filter Bag with residue (g)
f
S
= Initial Filter Bag (g)
D = Original wt of Diatomaceous Earth (g)
P = Protein of residue and bag (g)
A = Ash of residue and bag (g)
B = Blank (g)
= [(BR
1
+ BR
2
)/2] - P
B
- (A
B
- D
B
)
= [((f
BF1
- f
BS1
- D
B1
) + (f
BF2
- f
BS2
- D
B2
))/2] - P
B1
- (A
B2
- D
B2
)
BR
1
, BR
2
= Residue for duplicate blanks (g)
f
BF
= Final Blank Filter Bag (g)
f
BS
= Initial Blank Filter Bag (g)
P
B
= Protein of Blank Filter Bag (g)
A
B
= Ash of Blank Filter Bag (g)
D
B
= Original wt of Diatomaceous Earth in Blank Filter Bag (g)
X 100
X 100