ANKOM TDF Dietary - Manual (Page 90)

Operator’s Manual
pg. 90
Rev E 12/2/14
Appendix B – Instrument Out-of-use Procedure
The fluid lines can become obstructed when the instrument is left unused for one week or longer. To avoid fluid line
obstruction, execute the following procedure BEFORE the instrument is left unused for one week or longer.
1. Flush and purge the lines.
These instructions are available with additional pictures in Service Procedure 208
located at
www.ankom.com.
1.1 With the instrument turned on, press the Diagnostics button on the Touch Screen Display.
1.2 Press the Line Charge button on the Touch Screen Display. The following screen will be displayed.
1.3 Fill the Enzyme Containers on the left side of the instrument with Distilled Water. The enzyme lines are first flushed
with Distilled Water to avoid any precipitation from occurring due to an interaction between the enzymes and the
EtOH.
1.4 Press the Amylase button on the Touch Screen Display. This will draw water into the Amylase line. Do this a
second time to fully flush the line.
1.5 To flush the Protease and AMG lines, repeat step 1.4 using the Protease and AMG buttons on the Touch Screen
Display.
1.6 Disconnect Enzyme, HCl, EtOH78, EtOH95, Water, and Buffer containers. Store or dispose of all fluids properly.
1.7 Obtain the TDF70 Line Flush Tubing Assembly shown below. Note the nine connection points on the Tubing
Assembly.
1.8 Attach the Line Flush Tubing Assembly to the instrument and to the 78% EtOH Container as shown above.
1.9 Fill the 78% EtOH Container with 220ml of 78% EtOH.
1.10 Press the ALL button on the Touch Screen Display. This will draw 78% EtOH into all of the lines. Do this a second
time to fully flush the lines. This process will drain the 78% EtOH Container.
1.11 With the 78% EtOH Container empty, press the ALL button on the Touch Screen Display to purge the fluid from the
lines by sucking air into them. Press the ALL button a second time to fully clear the lines. There should now be
minimal fluid remaining in the lines. If a significant amount of fluid is observed, press the ALL button again until
the lines are clear (some droplets will remain). During the last line charge you should see no fluid being pumped
out to waste.
←
Line Charge
Amylase Protease AMG HCl
EtOH78 EtOH95 Water Buffer
ALL
Connect to HCl tube
Connect to right-side tubes
Connect to left-side (enzyme) tubes
Connect to 78% EtOH container