Jenway 6850 - Manual (Page 25)

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SECTION 7 – KINETICS
The kinetics measurement mode enables the absorbance or % transmittance of an active molecule to
be measured over a period of time; for example enzyme analysis of horseradish peroxidase. The
absorbance or % transmittance is measured at regular time intervals at a set wavelength over a period
of time. The results are plotted on a graph to show the change in absorbance or % transmittance over
time. Following sample measurement statistical analysis of all or part of the experiment can be
performed.
7.1 KINETICS MODE SCREEN
Select the Kinetics Measurement icon
on the toolbar. The Kinetics measurement
window will open.
7.2 METHOD SETUP
The kinetics scan settings can be edited by
selecting the settings icon in the toolbar.
The options that can be changed are:-
1. Scan wavelength
2. Scan time (max 100000s)
3. Scan Step (0.1, 0.2, 0.5, 1.0, 2.0,
5.0, 10.0, 30.0, 60.0s)
The required options can be entered directly or selected from the available options via the drop down
lists. Select OK to confirm.
7.3 SELECTING THE MEASUREMENT MODE
The required measurement mode can be selected from the available options of Abs and %T by
selecting the or icons on the toolbar.