Jenway 6850 - Manual (Page 29)

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SECTION 8 – DNA/PROTEIN
The DNA/Protein measurement mode allows the user to measure multi-wavelength absorbance ratios,
such as 260nm/280nm and 260nm/230nm, which are commonly used to estimate a protein or nucleic
acid sample’s purity. The mode also includes calculations that can be used to estimate the
concentration of the protein or nucleic acid sample.
Four commonly used protein assay methods are pre-loaded in this measurement mode. The available
protein assay methods are Direct UV, Lowry, Bradford, Biuret and BCA.
8.1 DNA/PROTEIN MODE SCREEN
Select the DNA/Protein Measurement icon
on the toolbar. The DNA/Protein measurement
window will open.
8.2 METHOD SETUP
The DNA/Protein method options are selected
from the Method drop down box. The methods
that are available are:-
1. 260/280
2. 260/230
3. UV Method
4. Lowry
5. BCA
6. Bradford
7. Biuret
8.2.1 Adjusting the Method Parameters
Select the DNA/Protein Measurement field to
edit and enter the required wavelength or
concentration factor. The displayed
concentration unit is selected from the Unit drop
down menu.