Jenway 6850 - Manual (Page 30)

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8.3 DETERMINATION OF NUCLEIC ACID CONCENTRATION
Select the required method parameters as in 8.2.
8.3.1 Sample Measurements
To quantify an unknown sample select the
Sample tab. Insert the sample cuvette into the
sample cuvette holder and the blank solution into
the reference sample holder.
Select the Start button.
A new window will open allowing the sample
name to be edited. The actual photometric
readings of the sample will be displayed in the
Readout window along with the results of the
calculations specified in the method settings.
Select OK to transfer the data to the sample
result table.
The sample result table will display the
measured absorbance values and the final
calculated concentration and ratio values.
8.4 DETERMINATION OF PROTEIN CONCENTRATION
Select the required method parameters as in 8.2.
8.4.1 Using a Concentration Factor or Pre-defined Calibration Curve
8.4.1.1 Entering Known Calibration Curve Constants
The known calibration curve constants can be
entered into the appropriate K0 – K2 fields
where:-
Concentration = (K2*X
2
)+ (K1*X) + K0
X = Photometric value of the sample