LaMotte SMART Spectro Spectrophotometer - Manual (Page 232)

PROCEDURE
Use universal sample holder
1. Preheat COD reactor to 150 ±2°C. Follow safety precautions.
2. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use a
1.0 mL pipet (0354) to add 5.0 mL of Deionized Water (5115PS). This is the
blank.
3. Remove cap from a *Total Phosphorus Acid Reagent Tube (4035). Use another
1.0 mL pipet (0354) to add 5.0 mL of sample water. This is the sample.
4. Use the 0.15 g spoon (0727) and a funnel (0459) to add one level measure
of *Digestion Reagent Powder (4036) to each tube. Tap funnel to dispense
powder completely. Cap tube tightly and shake until powder completely
dissolves.
5. Place the tubes in the COD reactor for 30 minutes.
6. At the end of the heating period, turn the reactor off. Carefully remove the tubes
from the reactor block and allow them to cool to room temperature.
7. Carefully remove the caps from the digested tubes. Use another 1 mL pipet
(0354) to add 2.0 mL of *Total Phosphorus HR Hydroxide Reagent (4037) to
each tube. Cap and invert to mix.
8. Use the 0.5 mL pipet (0353) to add 0.5 mL *Total Phosphorus HR Indicator
Reagent (4039) to each tube. Cap and invert to mix. Wait 7 minutes.
9. During the waiting period, press ON button until spectrophotometer turns
on.
10. Scroll to and select PROGRAMMED TESTS from menu.
11. Scroll to and select ALL TESTS (or another sequence containing
83 Phosphorus T HR) from PROGRAMMED TESTS menu.
12. Scroll to and select 83 Phosphorus T HR from the menu.
13. Wipe the vials with a damp towel to remove fi ngerprints and smudges. Wipe
with a dry towel.
14. Insert the blank tube into the chamber. Select SCAN BLANK. Remove the
blank tube from the spectrophotemeter.
15. Insert the sample tube into the chamber. Select SCAN SAMPLE. Record the
result as Total Phosphorus in mg/L PO
4
.
16. Press OFF button to turn the spectrophotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
SMART Spectro Test Procedures 2.11 PHOSPHORUS-TOTAL, High Range
Test P
rocedures