Section, Operating instructions, Opening and closing the lid – Iris Sample Processing StatSpin® ThermoBrite Denaturation and Hybridization User Manual

Page 10: Turning unit on, Run a denaturation and hybridization program

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6

Section

3

Operating Instructions

Opening and Closing the Lid

The platen may be hot. Use caution and check temperature on display before handling slides.
Improper precaution can cause a burn.

Depressions located on either side of the lid allow user to simply lift lid into position. The lid should
offer some resistance when opening. To close, reverse process. Assure front is completely down
and no obstructions prevent gasket seal from sealing on housing base.

Turning Unit On

The StatSpin ThermoBrite main power switch is located on the rear panel. Assure unit is plugged
into a grounded outlet. Move switch to on position. Instrument will beep to announce power has
been turned on. Main Menu will be displayed when the instrument has reached the default
temperature of 37°C..

Indicators on power switch: I = ON O=OFF

Run a Denaturation and Hybridization Program

Turn unit on and wait for the Main Menu screen. Cursor highlights

“Run a PGM” line.

Press “Enter” button to accept.

With the arrow keys scroll through program numbers 1 to 40 / program names. If no programs have been
saved advance to programming section of this handbook.

To accept, press “Enter” button.

Display will confirm PGM n

umber/name and Denat & Hyb times and temperatures. Cursor highlights “Run

PGM

” line. Press “Enter” button to accept.

Hyb Only

Denat & Hyb

Fxd Temp

PGM 01 Her2
82°C :05; 45°C 20:00
Run PGM
Main Menu

The display prompts to “Add Slides and Close Lid”. Before adding slides insert two Humidity Cards into
the inside slide lid. After strip insertion saturate with distilled water or equivalent (see Humidity Control
Cards
).

Cursor highlights “Start” line. Press “Enter” button to run the program.

Run a PGM
Edit a PGM
Create a PGM
Present Temp: 37°C

PGM 02 EBV
Hyb: 55°C 01:30
Run PGM
Main Menu

PGM 03 Appl
Fixed: 65°C
Run PGM
Main Menu

PGM 03 Appl

Add Slides - Close Lid

Start
Main Menu

PGM 02 EBV

Add Slides - Close Lid

Start
Main Menu

PGM 01 Her2

Add Slides - Close Lid

Start
Main Menu

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