Iris Sample Processing StatSpin® ThermoBrite Denaturation and Hybridization - Manual (Page 15)

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PGM 01 name
Enter: Temp Time
Denat: °C :00
Hyb: °C 00:00
(Reference Character Selection for additional information in regards to working with the keypad.)
Cursor will advance to “Denat Temp”. With numeric keypad enter a two-digit temperature value in
degrees Celsius (50-99ºC).
Cursor advances to “Denat Time”. With numeric keypad enter a two-digit time value in minutes (0 – 30)
Cursor advances to “Hyb Temp”. With numeric keypad enter a two-digit temperature value in degrees
Celsius (30-70ºC). For room temperature hybridization enter the two-digit value 00.
Cursor advances to “Hyb Time”. With numeric keypad enter a two-digit time value in hours (0 – 99)
followed by a two-digit value in minutes (0-59)
Display will now show entered program values. Cursor highlights “Enter to Accept” line.
Denat: 82°C :05
Hyb: 45°C 20:00
ENTER to Accept
STOP to Abort
Press “Enter” button to accept the program values; or press “Backspace” button to return to previous
screen to modify program values; or press the “Stop” button to abort.
Creating a Hybridization Only Program (Hyb Only)
From the Main Screen, use the arrow keys to move cursor to “Create a PGM” and press “Enter” button
to accept.
ThermoBrite will advance to the next available program number.
PGM 02 name
Enter: Temp Time
Hyb: °C 00:00
(Reference Character Selection for additional information in regards to working with the keypad.)
Cursor advances to “Hyb Temp”. With numeric keypad enter a two-digit temperature value in degrees
Celsius (30-70ºC). The instrument allows a temperature of 30
 C or ambient temp + 5  C (whichever is
higher) for the lowest hybridization temperature. For room temperature hybridization enter the two-digit
value 00.
Cursor advances to “Hyb Time”. With numeric keypad enter a two-digit time value in hours (0 – 99)
followed by a two-digit value in minutes (0-59)
Display will now show entered program values. Cursor highlights “Enter to Accept” line.
PGM 02 EBV
Hyb: 55°C 01:30
ENTER to Accept
STOP to Abort