Iris Sample Processing StatSpin® ThermoBrite Denaturation and Hybridization - Manual (Page 12)

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Turn unit on and wait for the Main Menu screen. Cursor highlights “Run a PGM” line.
Timer counts elapsed time. (Pressing “Enter” button will reset timer to zero).
PGM 03 Appl
Fixed Temp: 65°C
Reset Timer 01:18:10
End PGM/Main Menu
Use Arrow keys to move to “End PGM/Main Menu” line and press “Enter” button to accept.
Note: If ambient temperature is programmed the fan will continually cycle until the program is
aborted.
Note: The temperature can be increased or decreased as the unit is running by using the up/down
arrows from the “Fixed Temp” line.
Abort Program in Process
To end a program in process press “STOP” button, three beeps will sound. Use arrows to move cursor to
“Yes” line and press “Enter” button to accept. (Program will continue to run until “Yes” or “No” has been
accepted)
Note : The ThermBrite prompts, “Are You Sure?” This measure is to prevent accidental disruption of a
program in process.
ABORTING!!
Are You Sure?
No
Yes - Main Menu
Fan will turn on. If the slide temperature is above 37 ºC, the fan will cool to 37 ºC.
Slide Installation
Temperature uniformity across the heater is within 1ºC of set point across all slide positions.
ThermoBrite allows up to a maximum of 12 slides to be installed. When prompted simply lift lid and
load slides onto plate. Frosted edge of the slide should hang over edge. Move slide toward middle of
plate butting edge into marked positions in slide locator.
Be sure slides rest into slide locator before closing lid.
Humidity Cards
Located in the lid these cards act to prevent evaporation of sample from prepared slides. For best
results apply 8-10 mL of distilled or deionized water to each card for the first operation and for each
subsequent operation re-saturate with 3-10 mL to maintain moisture. Cards should be replaced
every 1-2 weeks as they will deteriorate over time and with use. In between runs the lid should
remain shut to keep the strips moist, however if the ThermoBrite remains unused for longer than 1
week remove strips, discard and open lid to dry unit out. In the event the cards completely dry out
they should be replaced and discarded. Replacement cards can be purchased for use with the
ThermoBrite.
To replace cards, lift lid and remove. Slide card into slot positions, allow tabs in lid to support cards.
CAUTION -Do not use paper towels or any other filter card in card positions. This may change the
humidity and may decrease the intensity of the probe, potentially causing erroneous results.