ChemoMetec NC-250 - Manual (Page 31)

991-0252 Rev. 1.1 February 2013 31
Plot Manager
The Plot Manager is used for evaluation and post processing of cell populations found in one or more image
files.
Post processing results are displayed in thumbnail-sized scatter plots and histograms arranged in rows and
columns, where each row comprises results for one particular image file.
The Plot Manager is specifically suited for batch processing of several image files as it can replicate
processing from one ‘Master’ row to all selected rows.
1. Master Row: Enter the number of the row that is to be used as the Master row.
2. Apply: This button replicates all processing from the Master row to all selected rows.
3. Mouse info field: The area to the right of the ‘Apply’ button displays information regarding the plot
coordinates and number of cells pointed to with the mouse.
button launches a Statistics window which lists detailed gating statistics for all visible polygons and
markers.
button launches a window to add Plot Manager files (*.cmpm) or post processing files (*.cmpp) to the
Plot Manager. Each added image will be appended as one new row.
Exceptions to this is when adding a *.cmpp file from the Master files directory or when a *.cmpm file holds
a master row. In these cases the file will be added to row number zero (i.e. as upper most row). In case row
zero already holds an image, the file will be appended.
Note, it is also possible to load image files (*.cm) and processed plot manger files (*.cmpp) into the Plot
Manager by dragging them from either the Browse tab in the main window or from the image browser.
There are two ‘save’ buttons in the Plot Manager. The ‘save’ button exists for each row and is placed in
the ‘Row window’ in the left part of the row. This button saves the post processing data i.e. gates, polygon,
quadrants and graphs associated with the individual sample. The second type of ‘save’ button at the top of
the window is always present (beside the button) and saves the current Plot Manager contents to a
*.cmpm file placed in a PM Files folder. This requires that all rows in the Plot Manager have been saved. All
unsaved rows may be saved by right clicking in the gray area in the top of the Plot Manager. The file name
is user defined via the edit field to the right of the ‘save’ button.