ChemoMetec NC-250 - Manual (Page 37)

991-0252 Rev. 1.1 February 2013 37
Add cells outside marker to image overlay(of shown): Selecting this option will add cells outside the
selected marker to the image overlay, causing these cells to be marked with a non-filled, enclosing square.
Note: Adding cells to the image overlay is cumulative when it is done from the same plot. When adding
cells to the image overlay from another plot, the overlay is first cleared. Consequently if you use this to
select both inside and outside cells you may assume that all cells in the image should be marked with an
enclosing square. This is however, not always the case. If certain cells have been excluded from the cell
population, e.g. all non-single cells, then these are not part of the histogram, and therefore will not be
included in the selected cells list.
Editing a Marker: A marker can be moved when the mouse curser is above the marker and is marked with
the move symbol . The left or right end of a marker can be moved when the mouse curser
is above the end of a marker and is marked with the move symbol
.
Scatter Plot in Plot Manager
The scatter plot is used to plot two user selectable parameters against each other together with protocol-
or user defined polygons or quadrants which marks, gates and counts cell populations.
1. Data source indicator: A small colored square in the upper left corner indicates the source of the
cell population (see further details in the section Row Window in Plot Manager).
2. Click the ‘delete plot’ button to remove a plot from the row. This will leave an empty area in
which you may, via right-click, insert another plot or simply delete the area.