Hoefer GD2000 - Manual (Page 17)

Step 2: Prepare the gel drying stack
The slab dryer accommodates both agarose and
polyacrylamide gels. The configuration of the gel
stack layers depends on the next processing step
and the thickness of the gel(s).
Drying between cellophane sheets
1
Immerse two sheets of porous cellophane in water.
2
Lay one cellophane sheet smoothly on top of the
filter paper liner. Carefully center the gel on the
cellophane. Cover the gel with the second sheet of
wet cellophane.
3
The stiff cover sheet produces a smooth gel surface
that reduces scan irregularities.
4
Check that the edges of all the sheets fall within the
recess of the platen. If necessary, trim the corners of
the sheets to fit within the recess.
5
Cover the stack with the silicone rubber sealing sheet.
•
p9
Fig 3. Gel drying stack for drying to
cellophane. Use this configuration
for gels to be scanned, imaged,
and stored.
Note: Cellophane provides
a transparent support for
densitometric scanning. Remove
all air pockets at every step of
this procedure to avoid scanning
distortions.
Drying between cellophane sheets
A filter paper liner and a stainless
steel screen lie under each stack.
A translucent rubber sealing sheet
lies on top.
The gel type and thickness
determine the cover sheet and
layers used around the gel.
rubber sealing sheet
stiff plastic cover sheet
porous cellophane
gel
porous cellophane
filter paper liner
stainless steel screen
rubber sealing sheet
stiff plastic cover sheet*
thin plastic wrap
gel thickness ≤ 1.5 mm
filter paper
filter paper liner
stainless steel screen
*not used with agarose gels
rubber sealing sheet
porous polyethylene cover sheet
gel thickness > 1.5 mm
filter paper
filter paper liner
stainless steel screen