Hoefer GD2000 - Manual (Page 20)

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Step 3: Set the temperature
The highest setting, 80 °C, can be used for many
types of gels for reliable, rapid drying. Use a
temperature of 50 °C for agarose gels. Poly-
acrylamide gels prepared for fluorography may
require a setting of 60 °C to protect the fluors.
Follow the manufacturer’s instructions. If your
gels tend to crack, slower drying at a lower
temperature setting may be indicated.
1
To set the temperature, make sure the red light
labeled “°C” is lit. If necessary, press the Time/Temp
key to toggle between the time and temperature
display.
2
Use the Adjust keys to change the temperature.
You can set the heater to any temperature from 40 to
80 °C or to OFF (room temperature).
Press the Up or Down key once briefly to change by
one degree. Press and hold the key down to count five
1-degree intervals, followed by 5-degree intervals.
When the display reads 40 °C, press the Down key
once to go to OFF. When the temperature is 80 °C,
press the Up key once to go to OFF.
Press the Up and Down arrows simultaneously to reset
the temperature to 40 °C. To use the vacuum at room
temperature, turn the heater off.
The platen begins to heat at the same time that
the vacuum pump starts— 10 seconds after you
have finished setting the time. A red light next to
the words “Heater On” indicates when the heat-
ing element is on.
Note: The melting temperature of
an agarose gel is dependent on
its concentration and properties.
The drying temperature should not
exceed the melting temperature.
We recommend a drying
temperature of 50 °C for most
agarose gels.
Note: When the heater attains the
temperature setting, the red heater
light goes off. The heater light
blinks when the heater comes on
to maintain the set temperature.