Hoefer GD2000 User Manual
Page 18
Drying thin or low-concentration polyacrylamide
gels (≤ 1.5 mm) and agarose gels on paper
1
Lay a sheet of filter paper on top of the filter paper
liner and position the gel on this sheet, taking care to
avoid trapping air beneath the gel.
2
Cover the gel with thin plastic wrap. Do not leave
wrinkles in the plastic wrap.
3
Use the stiff plastic cover sheet with polyacrylamide
gels but not with agarose gels.
4
Check that the edges of all the sheets fall within the
recess of the platen.
If necessary, trim the sheets to fit within the recess.
5
Cover the stack with the silicone rubber sealing sheet.
•
p10
Fig 4. Stacks for drying thin or
low-concentration polyacrylamide
gels and agarose gels to filter
paper for autoradiography.
rubber sealing sheet
stiff plastic cover sheet
porous cellophane
gel
porous cellophane
filter paper liner
stainless steel screen
rubber sealing sheet
stiff plastic cover sheet*
thin plastic wrap
gel thickness
≤ 1.5 mm
filter paper
filter paper liner
stainless steel screen
*not used with agarose gels
rubber sealing sheet
porous polyethylene cover sheet
gel thickness > 1.5 mm
filter paper
filter paper liner
stainless steel screen