Multichannel Systems HiClamp Manual - Manual (Page 13)

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3 HiClamp
3.1 Welcome to the HiClamp
The HiClamp is a fully-automated all-in-one solution for high-throughput functional secondary
screening of drug targets based on the standard Xenopus expression system. The HiClamp allows
an automated recording using the TEVC method unattended and over night.
Main features
 Compact and functional design, works fully computer-controlled.
 High performance TEVC amplifier, records current of up to  105 μA.
 Export features for further data evaluation.
 Integrated washing station with a peristaltic pump based four channel perfusion system.
 Non-destructive usage of test compounds.
 Small sample volumes (~200 μl).
Operation summary:
 The HiClamp functionality is based on the use of a novel system in which the oocyte is exposed
to a test solution by moving it physically into the solution of interest, whereas in a standard
system the solution is applied on the cell. The test solutions are disposed in a 96 microtiter plate
which is fixed on a table (in the left part). The oocyte is fixed in the support held (oocyte basket)
in the center of the automate and the table moves to immerge the cell in the desired solution.
The table moves in X, Y and Z direction under software control of very precise motors.
 The HiClamp software program is an “icon based” program and was designed to simplify the
user interface while maximizing the possibilities for electrophysiological recordings. No special
programming knowledge is required to use the HiClamp software.