Multichannel Systems HiClamp Manual User Manual

Page 14

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HiClamp Manual

8

 The program sequence is an ensemble of operations that are executed sequentially according

to a set of icons that are stored in the program sequence. Then, each icon corresponds to a given
procedure. For example, the oocyte icon will contain the type of message injected into the
oocytes, the number of cells available etc. At difference, the icon corresponding to the amplifier
will store the information controlling the amplifier gain, filter conditions, voltage clamp set up,
voltage steps etc. To access the content of a given icon the user double clicks on the icon stored
in the program sequence.

 After defining the recording protocol, simply start the recording sequence by mouse click.

The run will proceed automatically until finished or interrupted by the user.

The HiClamp software

Operate the HiClamp, collect and evaluate the data by using the HiClamp software. The easy-
to-use graphical user interface of the HiClamp software makes daily work with the HiClamp
quick and easy. Recording is started by a single mouse click. The HiClamp controls the run
for all 96 oocytes automatically. Thus the recording can go on overnight, unsupervised.

You can define different experimental setups not only for different well plates, but also
for specific selections of wells on the same plate. Several control features to save time and
compounds are provided.

Automated analysis features are included in the HiClamp software, but you can export the data
to your custom evaluation software as well. You can graph the data and generate reports with
the HiClamp software. The large amount of data generated by the HiClamp can be managed
with the included DataMerger software.

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