Multichannel Systems HiClamp Manual - Manual (Page 27)

TEVC Recording Protocol
21
Press on the Electrodes icon to open the Electrodes window.
Press on the "Down" button for slowly moving the electrodes down towards the oocyte basket.
Use the camera for optical control. Lower the electrodes progressively into the center of the
basket, just a little bit deeper than necessary to penetrate the oocyte. Note that the camera is
mounted on a rotating arm to facilitate the adjustments. If the electrodes are not centered use
the allen key (small hexagonal head screw) to move the position of the electrode holder base
plate.
If the electrodes are not correct aligned backward or forward use the icon Adjust Basket.
First move the basket in lateral and frontal direction by 30 units until the electrodes are in correct
position.
The adjustment is finished when the electrodes are lowered in the center of the basket in maximal
depth. This position of the electrodes is defined and saved when closing the window. Please use
the "Cancel" button to move the electrodes up and to restart the alignment procedure. Use
"Done" when you have found the right position. The position of the basket is stored in the file
BasketDefault.mat that is saved on your disk.
Important: Do not remove or edit the BasketDefault.mat file as this would destroy the calibration
parameters for the basket.