Easycal, User guide – BrandTech Scientific EASYCAL 4.0 User Manual
Page 31
EASYCAL
™
User Guide Version 4.0 · Status: June 2008
31
EASYCAL
™
User Guide
7.4 Calculations for Liquid Handling
Excerpt from ISO 8655/3, Table refers to 1013 hPa
Temperature Factor
Z
°C ml/g
15 1.0020
15.5 1.0020
16 1.0021
16.5 1.0022
17 1.0023
17.5 1.0024
18 1.0025
18.5 1.0026
19 1.0027
19.5 1.0028
20 1.0029
Temperature Factor
Z
°C ml/g
20.5 1.0030
21 1.0031
21.5 1.0032
22 1.0033
22.5 1.0034
23 1.0035
23.5 1.0036
24 1.0038
24.5 1.0039
25 1.0040
25.5 1.0041
Temperature Factor
Z
°C ml/g
26 1.0043
26.5 1.0044
27 1.0045
27.5 1.0047
28 1.0048
28.5 1.0050
29 1.0051
29.5 1.0052
30 1.0054
Table for Liquid Handling instruments
Factor “Z” [ml/g]
The following calculations must be carried out:
1. Mean weight:
x
_
=
x
1
+ x
2
+ x
3
+ x
4
+ x
5
+ x
6
+ x
7
+ x
8
+ x
9
+ x
10
10
2. Mean volume:
V
V
_
= x
_
·Z
⇒ For factor Z, see Table
3. Standard deviation:
s = Z ·
(x
1
- x
_
)
2
+ (x
2
- x
_
)
2
+ (x
3
- x
_
)
2
+ (x
4
- x
_
)
2
+ ...(x
10
- x
_
)
2
⇒ For factor Z, see Table
9
4. Accuracy:
A [%] =
V
_
- V
nominal value
· 100
V
nominal value
5. Coefficient of variation:
CV [%] = s · 100
V
_