Eppendorf Mastercycler pro - Manual (Page 49)

PCR run
Mastercycler pro
English (EN)
49
7 PCR run
7.1 Preparation
7.1.1 Selecting the sample tubes
The Mastercycler pro can be used with a great variety of sample tube and plate types. Consider the
following when selecting sample tubes, plates and lids:
• Only use sample tubes, plates and lids which have a heat resistance of up to approx. 120 °C.
• The sample tubes and plates must fit into the thermoblock bores free from play to achieve a good heat
transfer.
CAUTION! Unsuitable tubes can result in sample material escaping.
Unsuitable tubes may be so severely damaged that sample material is released.
 Use only tubes whose properties meet at least the requirements specified in these
operating instructions.
 This is especially important when working with infectious material.
CAUTION! Damage to heated lid from sharp-edged sample tubes or objects.
 Use only tubes whose properties meet at least the requirements specified in these
operating instructions.
 Do not use any sharp-edged or sharp objects on the heated lid.
 Extra caution should be exercised when cleaning so as not to damage the heated lid.
CAUTION! Temperatures on the inside of the heated lid may be higher.
If well caps consisting of thermally insulating material are used, the temperature on the inside
of the heated lid can be significantly higher than the temperature displayed.
 If you are using well caps consisting of thermally insulating material, for example foamed
material, make sure that it is heat-resistant up to at least 140 °C.