Waters PoraPak RXN Cartridges - Manual (Page 2)

[ Care and Use ManUal ]
PoraPak ™ RXN Cartridges and Bulk Care & Use 2
I. Common ApplICAtIons for CleAnup
Bases
Acids
Neutrals
Catch and Elute
Pass Through
Adsorption
PoraPak Rxn CX
PoraPak Rxn CX
PoraPak Rxn RP
TFA removal
Convert from high boiling
point solvent for bases
Catch and Elute
Catch and Elute
PoraPak Rxn CX
PoraPak Rxn CX
Fractionate sample
before Prep for bases
Remove water from post
column fraction for bases
Catch and Elute
Catch and Elute
PoraPak Rxn CX
PoraPak Rxn CX
Technique Recommended Product
Reaction Products
Reaction Components
Pre-and-Post
Preparative Column
Catch and Elute
In the “catch and elute” technique, the reaction mixture is loaded onto the cartridge; the analytes of interest are retained by the sorbent. A wash step follows to remove
additional reaction components from the cartridge. A strong solvent is used to elute the analytes from the cartridge. Sample concentration results when the final elution
volume is smaller than the load volume.
Pass Through
In the pass-through technique, the sorbent does not retain the compounds of interest, but does retain some class(es) of compounds and impurities, that can be discarded
with the column. Fractionation is achieved by the interaction between the impurities in the reaction mixture and the sorbent. No sample enrichment occurs during the
solid-phase extraction (SPE) step.
Adsorption
In the adsorption chromatography technique on a reversed-phase sorbent, the neutral compounds are moving down the chromatographic column in the presence of
organic solvent, albeit slowly, for small-percent organic washes. Load the reaction mixture in high water content
(75% water for example), wash to remove polar compounds, and then increase the organic content in large increments to elute neutrals. The increase is determined by
the hydrophobicity and MW of the compounds.