Waters PoraPak RXN Cartridges - Manual (Page 4)

[ Care and Use ManUal ]
PoraPak ™ RXN Cartridges and Bulk Care & Use 4
III. produCt GuIdelInes
The following tables should be used as guidelines for loading capacity, flow rates, and volumes of wash and elution solvents. These tables take into consideration the
dimension of the device and the bed volumes suggest appropriate flow rates and volumes for the condition, wash and elute steps.
The capacity is for the total of all compounds in the reaction that will be retained. For cation exchange, the capacity is for all bases in the reaction mixture including
product and reactants.
Consider smaller loads than maximum capacity guidelines or choose a larger device to avoid compound break through.
Flow rate guideline is meant to provide a flow for reaction loading step.
The reaction mix can be poured into the cartridge and allowed to flow by gravity. If the flow is too slow, simply add a small amount of pressure or vacuum to increase it.
If there is precipitate in the reaction vessel, rinse the vessel with more loading solvent and add to the top of the cartridge. During the washing steps, product trapped by
the precipitate may be dissolved and can be retained on the packed bed for later elution under the right solvent conditions.
This table provides guidelines for volumes used in the condition, wash and elution steps for the various sizes of PoraPak Rxn cartridges.
Maximum Capacity Guidelines PoraPak Rxn RP PoraPak Rxn CX
Configuration Retained Compounds Retained Base
6 cc-150 mg 15 mg/50 µmole 15 mg/50 µmole
6 cc-400 mg 40 mg/150 µmole 40 mg/150 µmole
20 cc-2 g 200 mg/350 µmole 200 mg/350 µmole
60 cc-5 g 500 mg/2000 µmole 500 mg/2000 µmole
Wash and Elution Volumes (mL)
Device Condition Wash Elute
6 cc-150 mg 3 mL 4 mL 4 mL
6 cc-400 mg 5 mL 10 mL 10 mL
20 cc-2 g 20 mL 20 mL 20 mL
60 cc-5 g 45 mL 45 mL 45 mL
Flow Rate Guidelines (mL/min)
6 cc cartridge 2 mL/min
20 cc cartridge 5 mL/min
60 cc cartridge 9 mL/min