Waters nanoACQUITY UPLC Columns - Manual (Page 4)

[ Care and Use ManUal ]
nanoACQUITY UPLC Column Care and Use 4
Problem
 If a clog or a blockage is created after the nanoACQUITY column
(e.g., at emitter) the pressure at the outlet of the column may rise
 The increase in pressure at the column outlet may create a temporary
leak between the column and the transfer tubing
 The most common source of post column clogs is a blocked
nanospray emitter
Note: Set high pressure limit on the nanoACQUITY system to approxi-
matively 1000 psi above maximum normal operating pressure.
Diagnosis 1
The pressure traces in the console window show an increase in
pressure during the analytical gradient (red arrows) between two
injections (highlighted by the red squares).
Notice the pressure during the trapping portions of the analysis did
not increase between injections (red arrows).
Diagnosis 2
This shows the increase in system pressure must originate after the
trap column.
Representative diagram of nanoACQUITY system with
attached nano trap and nano analytical column