Waters ACQUITY UPLC BEH Columns - Manual

CONT ENTS
I. INT RODUCTION
II. GET TING START ED
a. Column Connectors
b. Column Installation
c. Column Equilibration
d. eCord Installation
e. Initial Column Efficiency Determination
f. VanGuard™ Pre-Columns
III. COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
IV. COLUMN CLEANING, REGENERATING
AND STORAGE
a. Cleaning and Regeneration
b. Storage
V. INT RODUCING ECORD INT ELLIGENT
CHIP T ECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
VI. ADDITIONAL INFORMATION
a. Tips for Maximizing ACQUITY UPLC BEH
Column Lifetimes
b. Recommended Flow Rates and Backpressures for
Reversed-Phase ACQUITY UPLC BEH Columns
c. Getting Started with ACQUITY UPLC BEH
HILIC Columns
d. Getting Started with ACQUITY UPLC BEH
Amide Columns
I. INT RODUCTION
Thank you for choosing a Waters ACQUITY UPLC ® BEH Column.
The ACQUITY UPLC BEH packing materials were designed
specifically for use with the ACQUITY UPLC System and are
manufactured in a cGMP, ISO 9001 certified plant using ultra pure
reagents. Each batch of ACQUITY UPLC BEH material is tested
chromatographically with acidic, basic and neutral analytes and the
results are held to narrow specification ranges to assure excellent,
reproducible performance. Every column is individually tested and
a Performance Chromatogram and Certificate of Batch Analysis are
provided on the eCord™ intelligent chip.
ACQUITY UPLC Columns were designed and tested specifically for
use on the ACQUITY UPLC System. ACQUITY UPLC Columns will
exhibit maximum chromatographic performance and benefits ONLY
when used on the holistically-designed ACQUITY UPLC System since
the ACQUITY UPLC System and column were created and designed to
operate together. For these reasons, Waters cannot support the use
of ACQUITY UPLC Columns on any system other than an ACQUITY
UPLC System.
ACQUITY UPLC BEH Columns
[ CARE AND USE MANUAL ]