Care and use manual – Waters ACQUITY UPLC BEH Columns User Manual
Page 13
7. If acetone is used in one or more mobile phases, do not use
acetone as a sample diluent or needle wash solvent. Refer to
injection solvents section for sample diluent recommendations
and miscellaneous tip (#3) for needle wash solvent/purge
solvent recommendations.
8. Typical sample preparation suggestions for samples that contain
sugars/saccharides/carbohydrates:
a. Liquid Samples
i. Dilute with 50:50 ACN/H
2
O
ii. Filter using 0.45 µm or 0.22 µm syringe filter
(if necessary)
b. b. Solid Samples
i. Weigh out sample (~3 g) into 50 mL centrifuge tube
ii. Add 25 mL of 50:50 ACN/H
2
O and homogenize
(mechanically)
iii. Centrifuge at 3200 rpm for 30 minutes
iv. Collect supernatant and filter using 0.45 µm or
0.22 µm syringe filter (if necessary)
c. Depending on sample and/or analyte concentrations,
additional sample dilutions may be necessary.
d. More complex samples and/or lower analyte
concentrations may require additional sample
preparation steps and/or procedures such as solid phase
extraction (SPE).
e. Consider VanGuard BEH Amide pre-columns for UPLC
column protection.
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[ CARE AND USE MANUAL ]
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©2014 Waters Corporation. Produced in the U.S.A. November 2014 Rev H 715001371 KP-PDF