Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 11)

Recommended Focusing Parameters
7 cm 11 cm 17 & 18 cm 24 cm
Step 1: 250 V 30 min. 30 min. 30 min. 30 min.
Rapid Voltage Ramping
Step 2*:
4,000 V
60 min. — — —
8,000 V — 60 min. — —
10,000 V — — 60 min. 60 min.
Slow Voltage Ramping
Step 3*:
4,000 V
8–10 kV hrs. — — —
8,000 V — 15–20 kV hrs. — —
10,000 V — — 30–40 kV hrs. 40–60 kV hrs.
Rapid Voltage Ramping
* The maximum attainable voltage is determined by the combined final conductivity of the IPG
strip, sample, and rehydration buffer. A lower final voltage does not adversely affect resolution
provided that the set volt-hours are reached.
2.7 After Focusing Run is Complete
1. Remove the focusing tray from the PROTEAN IEF cell when the focusing run is complete by
lifting the tray by the handle with your thumbs.
Figure 10. Removal of Focusing tray from PROTEAN IEF cell.
2. Remove the cups from the focusing tray by inserting the enclosed forceps underneath the
connecting bridge, or by pinching the wall of the cup with forceps and lifting upwards. If a full
strip of cups (12) is used, you may have to lift the cups in a few places along the strip.
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