Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 2)

Table of Contents
S Se ec cttiio on n 1 1 IIn nttrro od du uc cttiio on n ........................................................................................................................................................................1 1
1.1 Overview..........................................................................................1
1.2 Introduction ....................................................................................1
1.3 Description ......................................................................................3
1.4 Specifications ..................................................................................4
S Se ec cttiio on n 2 2 IIn ns sttrru uc cttiio on ns s ............................................................................................................................................................................4 4
2.1 General Overview ............................................................................4
2.2 IPG Strips Rehydration Solution Preparation and
Strip Rehydration ............................................................................5
2.3 Transfer and Placement of Rehydrated IPG Strips in the
Focusing Tray ..................................................................................5
2.4 Placement of Movable Electrodes....................................................6
2.5 Positioning Sample Loading Cups and Loading Sample..................6
2.6 Focusing Conditions ........................................................................8
2.7 After Focusing Run is Complete ......................................................9
S Se ec cttiio on n 3 3 C Clle ea an niin ng g,, M Ma aiin ntte en na an nc ce e,, a an nd d C Ch he em miic ca all C Co om mp pa attiib biilliitty y ............................................1 10 0
3.1 Focusing Tray ................................................................................10
3.2 Movable Electrode Assemblies ......................................................11
3.3 Cups ............................................................................................11
S Se ec cttiio on n 4 4 G Ge en ne erra all G Gu uiid de elliin ne es s ffo orr S Sa am mp plle e P Prre ep pa arra attiio on n........................................................................1 11 1
S Se ec cttiio on n 5 5 T Trro ou ub blle es sh ho oo ottiin ng g ........................................................................................................................................................1 12 2
S Se ec cttiio on n 6 6 W Wa arrrra an ntty y ................................................................................................................................................................................1 13 3