Bio-Rad PROTEAN® IEF Cell Accessories User Manual
Page 5
1.3
Description
The Cup Loading Tray for use with the PROTEAN IEF cell consists of the following components:
Figure 2. Diagram of Cup Loading Tray components.
1. A twelve-cup strip that holds up to 100 µl of sample per cup and a twelve-cup strip that holds
up to 150 µl of sample per cup.
Note: For precise mechanical fit, a minimum of two cups is required. A single sample cup
provides an incomplete “stop” on the channel walls and the gel could be punctured.
2. A twelve channel focusing tray that accommodates IPG strips from 7cm to 24 cm.
3. Two movable electrode assemblies, each having 12 individual teeth with cavities which allow
mineral oil to flow through the entire channel of the focusing tray.
Electrode
Left handle
Tray base
Small (100 µl) sample cup
Electrode
Spring cup
Electrode
contact area
Electrode
contact area
Cup loading tray
Spring clip
Right handle
µ
l
Large (150 µl) sample cup
3