Bio-Rad PROTEAN® IEF Cell Accessories User Manual
Page 14
Section 5
Troubleshooting
Problem Cause
Solution
1. Sample is
Cup positioning is incorrect.
When positioning the cups, press down
leaking from
on the connecting bridge until it stops
the sample
on the channel wall of the focusing tray.
cups.
(See illustration on page 7 .)
The cut edge from the
Make sure to use a sharp cutting
connecting bridge contains
device such as scissors to evenly cut
a burr that prevents the
the connecting bridge. Inspect cut to
connecting bridge from
ensure that it does not contain any
proper positioning on the
burrs.
channel wall.
The cup is positioned in an
Make sure the IPG strips are rehydrated
IPG area that is not
evenly and thoroughly. See ReadyStrip
completely rehydrated.
IPG Strip Manual for correct rehydration
directions. Make sure the correct
volume is used for the selected IPG
strip. See table in Section 2.2.
The cup itself is
Replace cup.
malfunctioning.
2. Initial low or
Incorrect positioning of the
Make sure the electrodes are positioned
zero current
electrode assemblies.
directly on the IPG strips. See page 6
(no electrical
for correct positioning.
contact)
When using electrode wicks make sure
they are wetted properly and are placed
on the IPG strips and the electrode
assemblies are in contact with the
electrode wicks.
Make sure the movable electrode
assemblies make contact with the
electrode contact area on the focusing
tray.
Platinum wire on electrode
Inspect the platinum electrode wire to
holder has been
make sure it is intact. If in doubt, use a
compromised.
voltmeter to check for continuity.
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