Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 6)

Figure 3. Movable electrode assemblies.
1.4 Specifications
Material Polycarbonate
IPG strip size capacity 7 cm–24 cm
Tray Capacity Up to 12 IPG strips per run
Electrodes Platinum wire on movable electrode assembly
Inter-electrode distance User defined. Dependent on IPG strip length
IPG strip rehydration
Rehydration/equilibration tray specific to IPG strip length
Section 2
Instructions
2.1 General Overview
Isoelectric focusing with the Cup Loading Tray includes the following steps:
1. Prepare rehydration solutions and rehydrate IPG strips overnight in rehydration tray with
appropriate volume of rehydration buffer.
Note: The Cup Loading Tray is not intended for passive or active IPG strip rehydration. Please
use appropriate rehydration/equilibration tray.
2. Prepare sample.
3. Transfer the rehydrated IPG strips from the rehydration tray and place the strips gel side up
into the focusing tray’s channels.
Note: Reduced rehydration times result in incomplete rehydration of the IPG Strips causing an
imperfect seal between the sample cup and the IPG strip.
4. Optional. Place electrode wicks at anode and cathode.
5. Position the movable electrode assemblies on top of the strips at the anode and cathode
ends. Ensure the spring clips are in contact with the electrode contact areas.
6. Place the sample loading cups on the rehydrated IPG strips near the anode for basic pH
range and near the cathode for acidic pH range IPG strips.
7. Load sample in sample cup and overlay with mineral oil.
8. Overlay IPG strips in each channel with mineral oil.
9. Transfer tray to cooling platform in PROTEAN IEF cell and start isoelectric focusing.
4
Cavities
Teeth