Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 12)

Figure 11. Connecting bridge method. Figure 12. Pinching cup walls method.
3. Remove the IPG strips from the focusing tray. Carefully blot the IPG strip on clean, wetted
filter paper to remove excess oil and sample buffer. Equilibrate the IPG strips as required prior
to the 2nd dimension. See the ReadyStrip IPG Strip instruction manual for details.
Section 3
Cleaning, Maintenance, and Chemical Compatibility
Proper cleaning and maintenance of the Cup Loading Tray components will ensure the best
results while providing the longest life for the product.
3.1 Focusing tray
The focusing tray is made of polycarbonate. Several chemicals are not compatible with
polycarbonate: acetone, acetonitrile, benzene, chloroform, hexane, hydrazine, methylene chloride,
and acetaldehyde. This list is representative, but not exhaustive. The IEF sample preparation
reagents at the concentrations normally used for IEF, such as ampholytes, various detergents,
and reducing agents, including tri-butyl phosphine, have no effect on the trays.
Clean the focusing tray after each use. Use the nylon cleaning brushes included with the
PROTEAN IEF cell or a soft-bristled brush with hot water and an Alconox
® type of detergent
called Liqui-Nox ® , which is specifically designed for removing greasy substances, to remove all
residual mineral oil and sample solution. Rinse with DI water and air dry. Do not use ethanol to
wash or dry the focusing tray. Use of ethanol will craze or crack the tray and voids the warranty.
Note: Do not try to remove the mineral oil with any solvent, as most solvents will likely damage
the focusing tray and void the warranty.
10